Experience, educational needs and perception of dentine hypersensitivity of dental hygienists / 대한구강보건학회지

Objectives@#This study investigates dental hygienists’ experience, education, and awareness of dentin hypersensitivity. Its purpose is to provide a basis for the development of preventive procedures and educational materials for dentin hypersensitivity. @*Methods@#After distributing the questionnaire, Oral health characteristics of dental hygienists and the general public were investigated. In addition, the experience of dentin hypersensitivity according to general characteristics and the need for education were confirmed. The collected data were analyzed by frequency analysis, descriptive statistical analysis, unpaired t-test, and one-way ANOVA. @*Results@#There was no difference in the experience of dentin hypersensitivity according to oral health-related or general characteristics in the dental hygienist group. In terms of oral health, there was a difference in educational requirements for dentin hypersensitivity for ‘family support for oral health’. @*Conclusions@#The experience of dentin hypersensitivity in this study can be used as data for the development of a Korean dentin hypersensitivity experience questionnaire. The educational requirements and perception of dentine hypersensitivity will help develop educational materials from the patient’s perspective. Moreover, they will be essential for practical education on dentine hypersensitivity in dental hygiene and educational processes.

Nutrient Intake, the Concentrations of Leptin, Adiponectin, Cotisol & Insulin by the Body Fat Content of Women / 대한지역사회영양학회지

The aim of this study was to compare nutrient intakes, serum hormones (leptin, adiponectin, insulin), salivary cortisol and alpha-amylase of middle-aged women by the percentage of body fat (% fat). Subjects were assigned to 3 groups by body fat (%) group I (27.5%), group II (32.5%), group III (37.7%). WHR of group II (0.97) was significantly higher than of group I, III (0.95) (p < 0.05). Nutrient intakes were not different among 3 groups. Serum leptin levels of group III (16.53 microgram/ml) were higher than in group I (10.07 microgram/ml), group II (12.24 microgram/ml) (p < 0.05). Salivary cortisol levels of group II (0.39 microgram/dl) were higher than in group I (0.17 microgram/dl) and group III (0.15 microgram/dl) (p < 0.05). Adiponectin concentrations were negatively correlated with TAS (r = -0.29) and positively correlated with HDL cholesterol (r = 0.27). Insulin levels were negatively correlated with total cholesterol (r = -0.33), Zn intake (r = -0.31) and positively correlated with WHR (r = 0.31). The overall anthropometric indices showed positive relations with leptin levels. Salivary cortisol levels were positively corelated with WHR (r = 0.28), total cholesterol (r = 0.31), MDA (r = 0.29) and intakes of SFA (r = 0.35) and MUFA (r = 0.3). Salivary amylase levels were positively correlated with overall nutrient intakes (energy, CHO, fat, cholesterol. Fe, SFA, MUFA, Zn, Na, vitamin B2, r = 0.24-0.5) and was negatively correlated with HDL cholesterol (r = -0.34). These results suggested that 1) WHR would be a helpful index in the assessment of metabolic risk diseases. 2) Understanding of individual stress exposure should be considered in developing strategies for prevention and treatment of obesity.

Development and Evaluation of the Semi-quantitative Food Frequency Questionnaire to Assess Folate Intake in Women of Child-bearing Age / 대한지역사회영양학회지

The aim of this study was to develop and evaluate the simple semi-quantitative food frequency questionnaire (FFQ) for assessing folate intake in women of reproductive age. We developed a 30-item FFQ, and tested the reliability and validity in 97 women aged between 20 and 39 yrs using the FFQs and 24-h recalls, which were carried out twice, respectively. Assessing the reliability, the correlation coefficients were 0.53 (Spearman's) and 0.49 (Pearson's) for folate. There was no significant difference of folate intake between FFQ1 and FFQ2. 36.1% subjects were classified into the same quartile and 83.5% into the same or adjacent quartile divided by folate intake. On determining the validity, the mean folate intake obtained from the FFQ (FFQ1) and 24h-recalls (2-d) were 306.6 +/- 167.2 microg/d and 326.9 +/- 103.3 microg/d, respectively. There was no significant difference between folate intakes from the two methods. The correlation coefficients for folate were 0.24 (Spearman's) and 0.26 (Pearson's). 39.2% of the subjects were categorized into the same quartile and 70.1% were into the same or adjacent quartile. In addition, sensitivity (64.3%) and specificity (62.3%) were estimated to evaluate the adequacy of folate intake. These results suggest that this FFQ would be a useful and a valuable instrument to assess the intake of folate among the Korean women of child-bearing age.

Lifestyle, nutrient intake, iron status, and pregnancy outcome in pregnant women of advanced maternal age

The purpose of this study was to investigate how advanced maternal age influences lifestyle, nutrient intake, iron status, and pregnancy outcomes in pregnant women. The subjects of this study were 112 pregnant women who were receiving prenatal care at gynecologists located in Seoul. The subjects were divided into two groups according to their ages: those over age 35 were the advanced age group of pregnant women (AP) and those under age 35 were the young age group of pregnant women (YP). General factors, nutrient intakes, iron status, and pregnancy outcomes of the two groups were then compared. It was found that 72.5% of the YP group and 51.2% of the AP group had pre-pregnancy alcohol drinking experience; indicating that the YP group had more pre-pregnancy alcohol consumption than the AP group (P < 0.05). The only difference found in nutrient intake between the two groups was their niacin intakes which were 16.83 +/- 8.20 mg/day and 13.76 +/- 5.28 mg/day, respectively. When gestational age was shorter than 38.7 weeks, the average infant birth weight was 2.95 +/- 0.08 kg, and when gestational age was longer than 40 weeks, it averaged at about 3.42 +/- 0.08 kg. In other words, as gestational age increased, infant birth weight increased (P < 0.0001), and when maternal weight increased more than 15 kg, the infant birth weight increased significantly (P < 0.05). In conclusion, in order to secure healthy human resources, with respect to advanced aged women, it is necessary to intervene by promoting daily habits that consist of strategic increases in folate and calcium intake along with appropriate amounts of exercise.

Comparison of nutrient intake, life style variables, and pregnancy outcomes by the depression degree of pregnant women

The aim of this study was to analyse effects that the degree of depression have on the life style variables, nutrient intake, iron indices and pregnancy outcome. Subjects were 114 pregnant women who were receiving prenatal care at a hospital in Seoul. We collected data for general characteristics and lifestyle variables from general survey instrument and for depression score from the questionnaire on depression. Dietary intakes of subjects were estimated by 24 hour dietary recall method. Also we analysed iron indices and pregnancy outcomes. We classified subjects by 10 point, which was the average depression score, into two groups [Low depression score group (LS) : High depression score group (HS)]. As to the intakes of total calcium, plant-calcium, plant-iron, potassium, total folate and dietary folate, LS group was far higher than HS group (P < 0.05, P < 0.05, P < 0.01, P < 0.001, P < 0.05, and P < 0.01, respectively). As to pre-pregnancy alcohol drinking, LS group had 41.9% in non-drinker, which was far higher than 28% in HS group in non-drinker (P < 0.05). As for drinking coffee during pre-pregnancy, pregnant women who don't drink coffee in LS group took 43.6%, which was higher than 38% in HS group (P < 0.01). Regarding delivery type, the cesarean section in LS group (18%) was significantly lower than that in HS group (45%) (P < 0.01). Bivariate analysis showed that birth weight was significantly associated with the gestational age (P < 0.01). The pregnant women with higher depression score tended to have undesirable life habit, which might affect negative pregnancy outcomes. A better understanding of how depression and intake of nutrients work together to modulate behavior will be benefit nutritional research.

Nutritional and antioxidant status by skin types among female adults

This study was performed to analyze the relationship among sebum . hydration content of the skin and nutritional intake, serum antioxidant minerals and antioxidant enzymes, and lipid peroxide concentration in 50 female subjects in their 20s. The skin type was divided into Dry Skin, Mixed Skin, and Oily Skin, and the dry skin group was 14%, the mixed skin group was 56%, and the oily skin group was 30% of all subjects. The average age of the subjects was 20.54 +/- 1.43 years and BMI was 20.66. The average sebum content in each group was in the order of T-zone>forehead>chin>cheek. In case of the T-zone, a significant difference between the dry skin group and the oily skin group was observed, suggesting that the area is most sensitive to sebum content by skin type. Significant differences were not observed in energy and nutrient intakes by skin type. Serum concentrations of antioxidant minerals such as copper, manganese, zinc and selenium were not significantly different among the groups, but the dry skin group tended to be higher than the oily skin group. Serum catalase was significantly higher in the oily skin group (P < 0.05), and MDA was significantly higher in the mixed skin group (P < 0.05). The hydration of the cheek and serum zinc showed a negative correlation, and the sebum content of the cheek and GPx showed a significant negative correlation. The hydration of the forehead and serum copper showed a significant negative correlation, and the hydration of the forehead and GPx showed a significant positive correlation. The hydration of the chin and serum SOD showed a significant positive correlation. With these results, it is considered that the basic condition of nutritional status can affect the skin health.

Association between Work Departments and Carotid Intima-media Thickness in a Metal Manufacturing Plant / 대한산업의학회지

OBJECTIVE: To evaluate the relationship of work departments, carotid IMT, and plaque formation as predictors of cardiovascular disease in white-collar and blue-collar workers. METHOD: A total of 201 workers from a metal agricultural equipment manufacturing plant near Daegu were administered questionnaires to obtain information on their general characteristics, their work-related factors. In addition, the workers were given a clinical examination, which included a carotid artery ultrasonography. In total, 193 (96.0%) of questionnaires were completed and analyzed for this study. Multiple regression analysis and multiple logistic regression analysis were performed on the data for analysis. RESULT: Blue-collar workers had thicker carotid artery IMT when compared to white-collar workers. After adjustment, the carotid IMT was affected by age, working department, hypertension, and diabetes; plaque formation was affected by age, hypertension, total cholesterol, and excessive drinking. CONCLUSION: This study suggests that carotid IMT is associated with working department. Therefore, we need to examine the effect that occupational health services has on cardiovascular disease, focusing on the characteristics of each department.

The Use of Iron Supplements of Pregnant Women and Pregnancy Outcome / 대한지역사회영양학회지

It is known that Korean pregnant women take iron supplements at a higher than the recommended level. This study was designed to provide data on current iron intake levels both from food sources and supplement to better guide iron supplement use during pregnancy. We also explored associations of iron supplement intake levels with varioussociocultural factors and pregnancy outcomes. Dietary intakes of 510 pregnant women were assessed by a validated 102- item food frequency questionnaire, and information on types and amounts of nutritional supplement intakes were also attained. While dietary intake levels of most nutrients exceeded the KDRIs (Korea Dietary Reference Intakes: EAR: Estimated Average Requirements), folate fell short of the KDRIs. A total of 428 women (83.9%) reported to take iron supplement. The pregnant women were divided into the three groups (group I: Fe supplement intake or = 3rd pregnancy: 94.4 +/- 63.8 mg/day, p< 0.005). However, no significant difference was found between iron supplement intake levels and various pregnancy outcomes including birth weight, birth height, gestational age, weight gain during pregnancy, and jaundice. It is worrisome that iron intake by supplement use greatly exceeded the EAR, suggesting the need of appropriate guidelines for iron supplement intake during pregnancy. Thus iron overdose from supplements in pregnancy should be considered as a serious condition.

Dietary Intake, Serum Lipids, Iron Index and Antioxidant Status by Percent Body Fat of Young Females / 대한지역사회영양학회지

The aim of this study was to measure and compare nutrient intake, anthropometric measurements and serum indices by percent body fat as one of the index of obesity degree in female college students. Additionally we attempted to investigate percentile distribution of fat free mass index (FFMI) and fat mass index (FMI) for developing reference values for these two parameters. The subjects were 91 female college students who were classified to 4 groups according to the percentile of percent body fat (Group1: 25 th or = 75 th). The mean percent body fat and body mass index were 28.2%, 20.5 kg/m2 respectively. The mean energy intake was 1707 kcal(81% of KDRIs) and vitamin C, folate, Ca and Zn intake were 73.9%, 54.7%, 79.6%, 97.5% of KDRIs respectively. Most nutrient intake (energy, carbohydrate, cholesterol, fatty acid, Ca, Fe) of G4 was lower than that of G1, G2 and G3. Serum HDL-cholesterol concentration was significantly lower in G4 than G1, G2, G3 and it tended to increase as percent body fat decreased. LDL/HDL, AI of G4 were significantly the highest among the 4 groups and increased as percent body fat increased. The mean fat free mass index and fat mass index were 14.5 kg/m2, 6.0 kg/m2 respectively. The criteria of sarcopenic obesity which has been defined as under 25 th percentile of FFMI and below 75 th percentile of FMI were shown 12.8 kg/m2, 8.2 kg/m2 respectively in this study. In conclusion, we should continue to more systematically research on the studies of new obesity measurement which includes FFMI and FMI as one of the variables. And the public education for weight control that emphasizes both the understanding of body composition and the importance of nutrition balance is also required.

Dietary and Non-dietary Factors Related to Bone Mineral Density in Female College Students / 대한지역사회영양학회지

The purpose of this study was to investigate correlations between bone mineral density (BMD) and dietary and nondietary factors in female college students. The BMD of the subjects (n = 38) was measured using DEXA (Dual Energy X-ray Absorptiometry) at lumbar spine and three femoral sites including femoral neck, ward's triangle, and femoral trochanter. Three-day 24-hour dietary recall data were collected from each subject to assess consumption levels of nutrients and food groups. The mean (+/- SD) values of age, age of menarche, height, weight, body mass index (BMI), waist-to-hip ratio (WHR), fat mass, and % body fat of the subjects were 21.34 (+/- 1.73) years, 13.1 (+/- 1.2) years, 161.3 (+/- 5.0) cm, 53.7 (+/- 7.2) kg, 20.6 (+/- 2.6) kg/m2, 0.80 (+/- 0.04), 15.4 (+/- 4.4) kg, and 28.2 (+/- 4.7), respectively. The BMD values of lumbar spine, femoral neck, ward's triangle, and femoral trochanter as T-value were 1.150 +/- 0.13 g/cm2, 0.932 +/- 0.11 g/cm2, 0.850 +/- 0.13 g/cm2, and 0.721 +/- 0.10 g/cm2, respectively. The daily mean energy intake of the subjects was 1660.6 kcal. The intake levels of carbohydrate, calcium, iron, vitamin C, and folic acid were lower than the KDRIs, while those of fat, phosphorus, sodium, vitamin A, and vitamin B6 were higher than the KDRIs. Significantly negative correlation were detected between consumption of fat and oils and the BMD of all sites measured (p < 0.05). Potato and starch intake was negatively correlated to the BMD of femoral neck and word's triangle (p < 0.05). The intake of cereals was found to be negatively correlated to the BMD of ward's triangle (p < 0.05). There was also negative correlation of intake of soybeans and products with the BMD of lumbar spine (p < 0.05). Weight and muscle mass were positively correlated to the BMD of both lumbar spine and femurs (p < 0.01). Significantly positive correlations between BMI, and fat mass and the BMD of lumbar spine were observed (p < 0.001). Additionally fat mass was positively correlated to the BMD of femoral neck. WHR and % body fat were positively correlated to the BMD of lumbar spine (p < 0.05). Negative correlation was found between a serum calcium level and the BMD of femoral neck (p < 0.05). In summary non-dietary factors generally showed significant correlations with the BMD of lumbar spine, while several dietary factors showed significant correlations with the BMD of femurs.

Risk Factors Affecting the Health of Pregnant Women and Fetus / 대한지역사회영양학회지

The aim of this study was to determine the predictors of desirable pregnancy outcomes. The subjects were 795 pregnant women participating in the 2007 Mom and Baby Expo. They were grouped by gestational age: group I (3-12 wk: n = 95), group II (13-25 wks: n = 263) and group III (26-42 wks: n = 437). We collected data for general characteristics, sociocultural factors, life styles and nutrient intakes. We also collected pregnancy outcome data of 634 pregnant women including birth weight, maternal weight gain and gestational age. Dietary intakes of the subjects were estimated by Food Frequency Questionnaire. folate, iron and calcium intakes from foods of pregnant women were 88%, 79% and 58% of KDRIs, respectively. Bivariate analysis showed that birth weight was significantly associated with pre-pregnancy BMI, maternal weight gain, maternal age, gestational age and intakes of iron, potassium, vitaminB1, B6, fatty acids, MUFA. And also, bivariate analysis showed that maternal weight gain was significantly associated with pre-pregnancy BMI, maternal age, gestational age and intakes of energy, potassium. Further multivariate analyses suggest that vitaminB6 may be a significant predictor for low birth weight and energy intake and maternal age for maternal weight gain. Our findings suggest that dietary and lifestyle interventions during pregnancy can improve maternal and infant pregnancy outcomes. Prepregnancy weight control and intakes of energy and vitamin B6 need to be taken into considerations in developing strategic prenatal care programs to promote desirable pregnancy outcome.

Synthesis and evaluation of PDLs22 recombinant protein / 대한치주과학회지

Periodontal ligament (PDL) is the connective tissue located between the tooth root and alveolar bone. In a previous study, PDLs22 was isolated as a PDL-specific gene by using subtractive hybridization between cultured PDL fibroblasts and gingival fibroblasts. It was also suggested that PDLs22 plays important roles in the development, differentiation and maintenance of periodontal tissues. However, little is known about functional study of PDLs22 using recombinant protein in PDL fibroblast differentiation and periodontium formation. In this study, in order to produce the PDLs22 recombinat protein, PDLs22-expression vector were constructed and expressed its protein in various host cell and temperature conditions. The results were as follows: 1. PDLs22 protein was not strongly expressed in the induction system using pRSET-PDLs22 construct. 2. When the BL21(DE3) pLysS was used as a expression host, PDLS22 protein was strongly expressed in the induction system using pHCEIIBNd-PDLs22 construct. 3. The PDLs22 protein was recognized at a molecular weight of 28 kDa in western blots. 4. Almost of the expressed PDLs22 protein was not soluble and observed like as inclusion body. 5. The protein solubility was not improved after modification of induction time and temperature during PDLs22 protein production. In this study, the system for the PDLs22 protein production was connstructed. However, the results suggest that further studies will be needed to produce the considerable amount of PDLs22 recombinat protein, which can use for the periodontal regeneration.

Comparison of Nutrient Intake and Antioxidant Status in Female College Students by Skin Types / 대한지역사회영양학회지

The objective of this study was to evaluate whether nutrient intakes and serum biochemical indices are associated with the skin condition of humans. Anthropometric data, skin surface hydration, ceramide content, dietary intake of nutrients and serum lipids (total cholesterol, HDL-, LDL-cholesterol, TG, MDA: malondialdehyde, TAS: total antioxidant status) were obtained from 36 healthy young women. Subjects were classified into 2 groups {LM: Low Moisture Group (n = 20), HM: High Moisture Group (n = 16)} by forehead skin hydration. The results of this study were as follows: The average age, BMI, total body water, LBM, body fat%, WHR, forehead hydration, ceramide content were 20.2 yrs, 20.7, 28.8 l, 39.2 kg, 28.0%, 0.8, 44.3%, and 1.05 microgram/microgram protein, repectively. The intakes of SFA (saturated fatty acid), MUFA (monounsaturated fatty acid) in the HM group were significantly higher (p < 0.05) than in the LM group, but zinc intakes in the LM group were higher than in the HM group. Serum levels of TAS in the LM group (1.27 mmol/l) were significantly higher (p < 0.05) than that of in the HM group (1.20 mmol/l). Whereas other lipid levels were not significantly different, intakes of vitamin B6 and folate showed significant positive correlation with the forehead hydration (r = 0.447, r = 0.377). Intakes of calcium and phosphorus showed significant negative correlation with forearm ceramide content (r = -0.496, r = -0.485). Several associations between nutrient intakes and skin conditions were observed, indicating that changes in baseline nutritional status may affect skin health.

Dietary Intakes and Serum Lipids and Iron Indices in Obese Children / 대한지역사회영양학회지

This study was performed to compare nutrient intakes, diet quality and serum indices (TG, Total cholesterol, LDL-cholesterol, HDL-cholesterol, RBC, Hb, Hct, Serum iron, ferritin, MCV) of obese and normal weight children. The subjects were 149 children living in the Seoul and Gyeonggi areas. Each subject was assigned to one of such as normal weight group (15th < or = BMI percentile < 85th, n = 82) or obese children (85th < or = BMI percentile, n = 67) according to their percentile of BMI by The Korean Pediatric Society. Data on dietary intakes, body composition and serum indices were obtained. Differences of all the above variables were assessed. Energy, carbohydrate, fat, cholesterol, total fatty acid, SFA, MUFA, PUFA, phosphate, potassium, zinc, vitamin B1 and vitamin B6 intakes of girls in the obese group were higher than those of the normal weight group (p < 0.05). The intake of fiber was as insufficient as below 50% of KDRIs in both groups. Nutrient adequacy ratio of calcium, iron, phosphate, zinc and folate in obese boys were lower than those of normal weight boys (p < 0.05). Energy intakes of grain and milk and dairy food in the obese girl group (905.9 +/- 344.5 kcal, 210.9 +/- 166.4 kcal) were higher than those of normal weight girls (671.2 +/- 360.7 kcal, 184.0 +/- 103.5 kcal) (p < 0.05). HDL-cholesterol level of obese boys (52.7 +/- 6.3 mg/dL) was significantly lower than that of normal weight boys (65.3 +/- 15.6 mg/dL). Serum iron level of obese boys and girls (boys: 79.4 +/- 32.6 mg/dL, girls: 98.3 +/- 16.2 mg/dL) was significantly lower than those of normal weight children (boys: 104.8 +/- 38.6 mg/dL, girls: 106.7 +/- 28.9 mg/dL) (p < 0.05). These results suggest that there should be sensible food selection and more intakes of fruit and vegetable are needed to improve the nutritional status in obese children.

Body Mass Index, Dietary Intake, Serum Lipids and Antioxidant Status of Young Females / 대한지역사회영양학회지

Many epidemiological and observational studies show that distorted body image of normal body weight is widespread among Korean young females. This study was performed to evaluate the prevalence of overweight and underweight of young females and, to compare nutrient intakes, body composition, serum indices (lipids, MDA: Malondialdehyde, TAS: Total Antioxidant Status) with different BMI groups. The subjects were 75 university students in Seoul. We divided the subjects into 3 groups according to their BMI by IOTF guideline (UW: underweight group; BMI or = 23.0). Data on dietary intakes, body compositions and serum indices were obtained in 3 groups. Differences on all of the above variables were assessed by body weight groups. Using IOTF guidelines, the prevalence of overweight and underweight in young females were 23%, 61%, 16% respectively. beta-carotene and vitamin A intake of UW were significantly higher than that of NW (p < 0.05). Fiber intakes of NW was significantly higher than that of UW (p < 0.05). But intakes of energy and the other nutrients were not significantly different among BMI groups. Calcium and folate intakes were 75%, 61% of KDRIs. Serum TGs were significantly higher in OW than that of NW, UW (p < 0.05), but level of MDA and TAS were not significantly different. The association of overweight and low intake of beta-carotene and vitamin A may be one of many factors predisposing obese females to a high risk of oxidative stress later in life. This requires urgent nutritional intervention programs involving enough intake of fruit and vegetables, with modification of inappropriate dietary habits.

Selenium, Manganese, Copper and Zinc Contents in Serum of Patients with Cervical Intraepithelial Neoplasia / 대한지역사회영양학회지

The purpose of this study was to investigate the implications of dietary intake and the level of serum micronutrients (Se, Mn, Cu, Zn), lipid peroxidation, and antioxidant capacity in Korean women with cervical intraepithelial neoplasia (CIN). From October 2002 to March 2003, 50 patients diagnosed with CIN (confirmed with colposcopy directed biopsy) and 50 patients without any cervical disease as the control group were enrolled in the study at the Department of Gynecology Cancer Center at Samsung Cheil Hospital. Animal fat intake in CIN group was significantly higher than that of the control group, but plant protein intake in the CIN group was significantly lower than that of the Control group. Energy and zink intakes were similar in the two groups. The serum concentration of antioxidant minerals in the CIN group were not signifi-cantly different from the control group. The total radical trapping antioxidant potential concentration of plasma was sig-nificantly lower in the CIN group (1.12 mM) than in the control group (1.25 mM)(p < 0.05). But MDA (malondialde-hyde) of serum was significantly higher in the CIN group (7.60 mM) than in the control group (4.99 mM)(p < 0.005). The serum selenium concentration of the control group showed significant positive correlation with the MDA level (r = 0.311). These findings are suggestive of protective roles for healthy dietary habit including increasing antioxidant nut-rients and decreasing intake of fat.